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rp4 orit site  (Addgene inc)


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    Addgene inc rp4 orit site
    Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.
    Rp4 Orit Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rp4+orit+site/pmc06866695-151-58-27?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    rp4 orit site - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Midbiotics: conjugative plasmids for genetic engineering of natural gut flora"

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora

    Journal: Gut Microbes

    doi: 10.1080/19490976.2019.1591136

    Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.
    Figure Legend Snippet: Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.

    Techniques Used: Plasmid Preparation, Sequencing



    Similar Products

    rp4 orit site  (Addgene inc)
    90
    Addgene inc rp4 orit site
    Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.
    Rp4 Orit Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rp4+orit+site/pmc06866695-151-58-27?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    rp4 orit site - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.

    Journal: Gut Microbes

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora

    doi: 10.1080/19490976.2019.1591136

    Figure Lengend Snippet: Bacterial strains and plasmids used in the experiments and the spacer sequences of pCRISPR plasmid. Only the resistance genes relevant to the experiments are mentioned here.

    Article Snippet: In this study, the so-called midbiotic system consists of the conjugative RP4 blaTEM−2∆172−714 plasmid (delivery plasmid) and mobilizable pCas9 plasmid (pCRISPR plasmid, a gift from Luciano Marraffini, Addgene plasmid # 42876) encoding the S. pyogenes CRISPR/Cas9 system with crRNA(s) targeting conservative sites of different beta-lactamase resistance genes in ESBL plasmids ( ). pCas9 was made mobilizable by cloning RP4 oriT site , (50980–51793 bps, amplified with primers RP4oriT-F and RP4oriT-R, Supplementary Table 1) into pCas9 digested with SalI (ThermoScientific; Waltham, Massachusetts, United States) into region spanning 7377–7486 bps.

    Techniques: Plasmid Preparation, Sequencing